Mammalian Cells Change Volume during Mitosis
نویسندگان
چکیده
منابع مشابه
Mammalian Cells Change Volume during Mitosis
Using single cell-imaging methods we have found that the volume of adherent cells grown in culture decreases as the cells rounds when it enters mitosis. A minimal volume is reached at metaphase. Rapid volume recovery initiates before abscission as cells make the transition from metaphase to cytokinesis. These volume changes are simultaneous with the rapid surface area decrease and recovery obse...
متن کاملOptical volume and mass measurements show that mammalian cells swell during mitosis
The extent, mechanism, and function of cell volume changes during specific cellular events, such as cell migration and cell division, have been poorly studied, mostly because of a lack of adequate techniques. Here we unambiguously report that a large range of mammalian cell types display a significant increase in volume during mitosis (up to 30%). We further show that this increase in volume is...
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Chinese hamster cells in the mitotic and G(1) phases of the growth cycle were incubated for 30 or 60 min in suspension tissue culture and pulse-labeled with tritiated uridine. After appropriate chases, washes, and extractions, it was found that all incorporation into the nucleic acid may be accounted for by those cells in interphase. An average of 410 counts was found for incorporation into the...
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The Ran GTPase plays a central function in control of nucleo-cytoplasmic transport in interphase. Mitotic roles of Ran have also been firmly established in Xenopus oocyte extracts. In this system, Ran-GTP, or the RCC1 exchange factor for Ran, drive spindle assembly by regulating the availability of 'aster-promoting activities'. In previous studies to assess whether the Ran network also influenc...
متن کاملUsing cultured mammalian cells to study mitosis.
Diverse cell types have been used to study various aspects of mitosis. Early investigators focused primarily on cells that were suited to morphological studies. More recently, experimental systems have been developed to study both morphology and the molecular basis of chromosome motion and cell-cycle regulation. This article briefly reviews cell types that have been used to study mitosis in liv...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2008
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0001477